Figure 4.

Immunolocalization of DXR in maize roots visualized by confocal laser scanning micrographs of root sections. A, Cortex cell of a nonmycorrhizal root after incubation of root section with affinity-purified antiserum against 6×-His-ZmDXR (1:1,000); B, Arbusculated cell (anti-6×-His-ZmDXR, 1:1,000); C, Arbusculated cell after incubation with preimmune serum (1:1,000). The green fluorescence obtained with the anti-DXR/Alexa488 stain is indicative of the presence of DXR, whereas fungal structures are shown in red after staining with wheat germ agglutinin coupled to tetramethyl rhodamine isothiocyanate (WGA-TRITC). DNA is represented by blue color resulting from 4′,6′-diamidine 2-phenyl indole (DAPI) staining. For each micrograph, projections of 25 optical sections are superimposed. Bars = 5 μm.