ZmPRPL35 expression in wild-type and mutant tissues. Expression of ZmPRPL35 (A, C, and E) and a Gapdh control (B, D, and F) in various tissues of the maize plant (A and B), at various stages of kernel development (C and D), and in mutant and wild-type tissues (E and F) was monitored by RT-PCR. Equal amounts of total RNA were used for the reactions. PCR products were separated by gel electrophoresis, blotted onto nylon membranes, hybridized with a ZmPRPL35 probe, and detected by autoradiography. E, Immature ear; E16, wild-type embryo at 16 DAP; E24, wild-type embryo at 24 DAP; E30, wild-type embryo at 30 DAP; e30, mutant embryo at 30 DAP; En16, wild-type endosperm at 16 DAP; en16, mutant endosperm at 16 DAP; KX, kernel at X DAP; Lm, mature leaf; Ly, young leaf; Ov, ovule; R, root; Sd, dark-grown seedling; Si, silk; Sl, light-grown seedling; St, stem; T, tassel.