FIGURE 4. Gradients of lymphatic fluid stasis regulate the expression of PPAR-γ.
A. PPAR-γ+ cells/high powered field (HPF) cell counts in the various regions of the tail relative to the zone of lymphatic obstruction. Note significant increase in the number of PPAR-γ+ cells/HPF in distal sections (***p<0.001).
B, C, D. Representative high (40x) images of cross-sectional regions of the mouse tail relative to the zone of lymphatic obstruction stained for PPAR-γ. Note increased number of positively stained cells in the distal regions. Also note staining of mononuclear cells in subcutaneous fat in distal regions (red arrows).