Skip to main content
. 2004 Feb;134(2):664–675. doi: 10.1104/pp.103.033365

Figure 2.

Figure 2.

Yeast complementation of PvUPS1. Growth assays were performed with the yeast strains dal4/dal5 (A) and 22Δ8AA (B). A, dal4/dal5 that is deficient in allantoin transport was transformed with PvUPS1/pDR196, AtUPS1, and AtAAP2 in pDR196 (Fischer et al., 1995; positive controls) or pDR196 only (negative control) and were grown on media containing allantoin as sole nitrogen source. B, To test if PvUPS1 mediates growth of yeast cells on amino acids, 22Δ8AA (deficient in citrulline, Pro, Arg, Asp, and GABA transport) expressing PvUPS1/pDR196, AtUPS1/pDR196, AtAAP2/pDR196, or the empty pDR196 vector were grown on media supplemented with citrulline, Pro, Arg, Asp, and GABA as sole nitrogen source (here shown only yeast complementation on citrulline and Pro).