Figure 1.

Thermodynamic cycle illustrating the decomposition of the standard binding free energy [Eq. (13)]. R_solv is the solvated receptor, L_solv,C○ (upper left) is the ligand in solution at concentration C○, L_solv,site (lower left) is the ligand solvated sequestered in the binding site, L_gas,site (lower right) is the ligand in the gas phase in a volume equal to the binding site volume, and RL_solv is the solvated complex.