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. Author manuscript; available in PMC: 2013 Sep 14.
Published in final edited form as: Cell. 2012 Sep 14;150(6):1264–1273. doi: 10.1016/j.cell.2012.08.020

Figure 3. Myelination, Synapse Formation and Expression of Neurotransmitters.

Figure 3

(A–B) Graft-derived, GFP-labeled axons are myelinated in many cases, (red, myelin-associated glycoprotein, MAG, confirmed by electron microscopy (T, transplanted, GFP-labeled axon). (C) GFP-expressing axon terminals are closely associated with host MAP-2-expressing neurons and dendrites, and (D) host Tuj1-expressing neuronal somata. (E) A z-stack image triple labeled for GFP, synaptophysin (Syn, inset), and ChAT, indicating co-association of graft-derived axons with a synaptic marker in direct association with host motor neurons (arrowhead indicates one of several examples). (F) Electron microscopy confirms that DAB-labeled GFP-expressing axon terminals form synapses (arrows) with host dendrites. Arrowhead indicates a separate, host-host synapse. (G–H) Expression of vGlut1/2 or GAD65 by GFP-labeled axons (arrows and insets) in close association with host motor (ChAT-labeled) neurons. Scale bar: A, 3μm; B, 200nm; C–E, 8μm; F, 200nm; G, 7μm; H, 6μm.