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. 2004 Feb;134(2):684–693. doi: 10.1104/pp.103.033399

Figure 4.

Figure 4.

Identification of AtSUC3 promoter activity or AtSUC3 protein in various sink cells and tissues of Arabidopsis. A, GUS-histochemical staining of an anther from an AtSUC3-promoter::GUS plant showing blue GUS staining in the pollen grains of a still closed anther. B, GFP fluorescence in pollen grains still attached to an open anther from an AtSUC3-promoter::GFP plant (EF, Epifluorescence). The yellow fluorescence results from cell wall phenolics deposited during anther maturation. C, GFP fluorescence in the tube of a germinating pollen from an AtSUC3-promoter::GFP plant (CLSM). D, Immunolocalization of AtSUC3 protein in the pollen tube of an Arabidopsis wild-type plant. E, GFP fluorescence in a pair of guard cells from an AtSUC3-promoter::GFP plant (CLSM). F, GUS-histochemical staining of two pairs of guard cells from an AtSUC3-promoter::GUS plant. G, GFP fluorescence in the trichomes from an AtSUC3-promoter::GFP plant (EF). H, GUS-histochemical staining in the detached trichome of an AtSUC3-promoter::GUS plant. I, GFP fluorescence in the root tip of an embryo from an AtSUC3-promoter::GFP plant (EF). J, GUS-histochemical staining in the root tip of an embryo from an AtSUC3-promoter::GUS plant. K, GFP fluorescence in the tip of an emerging lateral root from an AtSUC3-promoter::GFP plant (CLSM). L, GUS-histochemical staining in the tip of an emerging lateral root from an AtSUC3-promoter::GUS plant. M, GFP fluorescence in the stipules of an AtSUC3-promoter::GFP plant (CLSM). N, GUS-histochemical staining in the stipules of an AtSUC3-promoter::GUS plant. O, GFP fluorescence in the suspensor of an ovule from an AtSUC3-promoter::TM-GFP plant (CLSM). P, GFP fluorescence in the seed coat of an developing ovule from an AtSUC3-promoter::TM-GFP plant (CLSM). Scale bars = 100 μm in A, B, H, I, and J, = 20 μm in C and O, = 10 μm in D, = 8 μm in E and F, = 200 μm in G, and = 80 μm in K, L, M, N, and P.