Figure 2. M-group consensus peptides are recognized by PBMC from HIV-1-infected patients.
IFN-γ ELISPOT assay was performed to evaluate T-cell responses against the 27 M-group consensus peptides by stimulating PBMC from patients infected with different variants of HIV-1, which are represented by colored symbols. A) IFN-γ secretion against Pol peptides. B) IFN-γ secretion against Gag peptides. C) IFN-γ secretion against Vif, Rev, Vpr, Vpu and Nef peptides. B subtype-infected patients (n = 14), BC recombinant-infected patient (n = 1), BF recombinant-infected patients (n = 9), F subtype-infected patient (n = 1). Dotted lines represent IFN-γ ELISPOT cutoff, which is ≥50 spots/106 cells. Only positive responses are shown in the graphs.