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. 2012 Sep 18;7(9):e45225. doi: 10.1371/journal.pone.0045225

Figure 7. ST2 deletion leads to inflammatory phenotype of APC after EAE induction.

Figure 7

Mononuclear cells were isolated from lymph nodes of ST2−/− and WT BALB/c mice 4, 7 and 9 days after immunization and analyzed by flow cytometry. (A) Percentages of subpopulation of dendritic cells, CD11c+CD11b+CD8−, in the lymph nodes 4, 7 and 9 days after immunization are presented. (B) Expression of markers of activation, CD86 and MHC II, on dendritic cells was performed at day 4. Histograms were gated on CD11c+CD11b+CD8− cells. (C) Percentages of CD11c+CD11b+CD8− cells positive for IL-1, IL-12 and IL-6 in lymph nodes 4 days after immunization is presented. Data are presented from 3 independent experiments as the mean+SD of representative experiment (n = 23 in both groups). Statistical significance was tested by Student’s t-test.