Skip to main content
. 2012 Sep 18;7(9):e45456. doi: 10.1371/journal.pone.0045456

Figure 6. EPO and gene silencing of PRAS40 reduce caspase-3 activation during OGD.

Figure 6

(A) EPO (10 ng/ml) was applied 1 hour prior to OGD in SH-SY5Y cells. Western blot analysis was performed for the cleaved fragments of caspase 3 with a antibody that identifies both the 19 kDa and 17 kDa fragments of caspase 3 hours following OGD exposure. PRAS40 siRNA transfection significantly reduced the expression of caspase 3 cleaved fragments during OGD exposure and during EPO administration with OGD exposure. (B) Quantitative results of the band density of the western blot analysis for caspase 3 fragments show that PRAS40 siRNA transfection significantly reduced the expression of caspase 3 cleaved fragments during OGD exposure and during EPO administration with OGD exposure (*P<0.01 vs. untreated control; † P<0.01 vs. OGD treated alone). Scrambled siRNA transfection did not alter the expression of the caspase 3 fragments during OGD alone or during EPO with OGD. PRAS40 siRNA transfection did not significantly alter caspase 3 fragment expression in untreated cells when compared to cells receiving no treatments. Each data point represents the mean and SD from 3 experiments.