Figure 3.

Synthesis of (1→3),(1→4)-β-glucan cellotriose and cellotetraose units upon detergent treatment. CHAPS concentrations are below CMC, and experiments were performed with and without pretreatment with proteinase K at concentrations indicated. Molar equivalent amounts of (1→3),(1→4)-β-glucan synthesized as estimated from radioactivity incorporated into the cellotriose (trimer) and cellotetraose (tetramer) units quantified as described in Figure 1. Control activities of (1→3),(1→4)-β-glucan synthase in 0%, 0.1%, and 0.2% (w/v) CHAPS are 0.70, 1.12, and 0.40 pmol μg protein^–1 after 1.5-h incubations with 250 μm UDP-Glc in RB, respectively.