Figure 9. Lateral ventricle DiI injections do not reach the 4^th ventricle of JAM-C^−/− mice with symptomatic hydrocephalus.

A Injection scheme for DiI is shown. B DiI (red) distribution in the ventricular system in sagittal sections from a C57BL/6 wild-type and a hydrocephalic JAM-C^−/− C57BL/6 mouse is shown. Nuclear staining (DAPI) is shown in blue, unspecific fluorescence in green. LV = lateral ventricle; asterisk = 3^rd ventricle; arrows point to 4^th ventricle. C–F DiI distribution within the lateral ventricles. G–J DiI distribution within the 3^rd ventricle. Note that in J (JAM-C^−/− score 1) the 3^rd ventricle contains little or no DiI. K–N DiI distribution within the 4^th ventricle. Note that in N (JAM-C^−/− score 1) the 4^th ventricle contains no DiI.