Table 1. FRET interactions between CENP-O class proteins.

EGFP fusion	mCherry fusion	p	FRET
EGFP-CENP-P	mCherry-CENP-O	<0.001	++
EGFP-CENP-P	CENP-O-mCherry	0.093	−
CENP-P-EGFP	mCherry-CENP-O	<0.001	++F
CENP-P-EGFP	CENP-O-mCherry	<0.001	++
EGFP-CENP-Q	mCherry-CENP-O	<0.001	++
EGFP-CENP-Q	CENP-O-mCherry	<0.001	++
EGFP-CENP-Q	mCherry-CENP-P	<0.001	++
EGFP-CENP-Q	CENP-P-mCherry	0.724	−
EGFP-CENP-Q	mCherry-CENP-Q	<0.001	++
CENP-Q-EGFP	CENP-Q-mCherry	<0.001	++
CENP-Q-EGFP	mCherry-CENP-P	<0.001	++F
EGFP-CENP-U	mCherry-CENP-O	<0.001	++
EGFP-CENP-U	CENP-O-mCherry	0.655	−
EGFP-CENP-U	mCherry-CENP-P	0.003	+
EGFP-CENP-U	CENP-P-mCherry	<0.001	++
EGFP-CENP-U	mCherry-CENP-Q	<0.001	++
EGFP-CENP-U	mCherry-CENP-R	<0.001	++
EGFP-CENP-U	CENP-R-mCherry	<0.001	++
CENP-U-EGFP	mCherry-CENP-P	<0.001	++
EGFP-CENP-B	CENP-Q-mCherry	<0.001	++
EGFP-CENP-O	mCherry-CENP-K	<0.001	++
CENP-O-EGFP	mCherry-CENP-K	0.004	+
EGFP-CENP-R	mCherry-CENP-K	<0.001	++
CENP-R-EGFP	mCherry-CENP-K	<0.001	++
EGFP-CENP-U	mCherry-CENP-K	<0.001	++
CENP-U-EGFP	mCherry-CENP-K	0.167	−
EGFP-CENP-U	mCherry-CENP-U	<0.001	++
CENP-N-EGFP	mCherry-CENP-K	<0.001	++

The FRET pair EGFP-mCherry is used. “F” indicates that for these fusions FRET was detected also by FLIM. ++: strong FRET, +: weak FRET, −: no FRET.