Table 1. FRET interactions between CENP-O class proteins.
EGFP fusion | mCherry fusion | p | FRET |
EGFP-CENP-P | mCherry-CENP-O | <0.001 | ++ |
EGFP-CENP-P | CENP-O-mCherry | 0.093 | − |
CENP-P-EGFP | mCherry-CENP-O | <0.001 | ++F |
CENP-P-EGFP | CENP-O-mCherry | <0.001 | ++ |
EGFP-CENP-Q | mCherry-CENP-O | <0.001 | ++ |
EGFP-CENP-Q | CENP-O-mCherry | <0.001 | ++ |
EGFP-CENP-Q | mCherry-CENP-P | <0.001 | ++ |
EGFP-CENP-Q | CENP-P-mCherry | 0.724 | − |
EGFP-CENP-Q | mCherry-CENP-Q | <0.001 | ++ |
CENP-Q-EGFP | CENP-Q-mCherry | <0.001 | ++ |
CENP-Q-EGFP | mCherry-CENP-P | <0.001 | ++F |
EGFP-CENP-U | mCherry-CENP-O | <0.001 | ++ |
EGFP-CENP-U | CENP-O-mCherry | 0.655 | − |
EGFP-CENP-U | mCherry-CENP-P | 0.003 | + |
EGFP-CENP-U | CENP-P-mCherry | <0.001 | ++ |
EGFP-CENP-U | mCherry-CENP-Q | <0.001 | ++ |
EGFP-CENP-U | mCherry-CENP-R | <0.001 | ++ |
EGFP-CENP-U | CENP-R-mCherry | <0.001 | ++ |
CENP-U-EGFP | mCherry-CENP-P | <0.001 | ++ |
EGFP-CENP-B | CENP-Q-mCherry | <0.001 | ++ |
EGFP-CENP-O | mCherry-CENP-K | <0.001 | ++ |
CENP-O-EGFP | mCherry-CENP-K | 0.004 | + |
EGFP-CENP-R | mCherry-CENP-K | <0.001 | ++ |
CENP-R-EGFP | mCherry-CENP-K | <0.001 | ++ |
EGFP-CENP-U | mCherry-CENP-K | <0.001 | ++ |
CENP-U-EGFP | mCherry-CENP-K | 0.167 | − |
EGFP-CENP-U | mCherry-CENP-U | <0.001 | ++ |
CENP-N-EGFP | mCherry-CENP-K | <0.001 | ++ |
The FRET pair EGFP-mCherry is used. “F” indicates that for these fusions FRET was detected also by FLIM. ++: strong FRET, +: weak FRET, −: no FRET.