Table 3. Long term FRAP results for the CENP-PORQU proteins.
Cell cycle | CENP-O | CENP-P | CENP-Q | CENP-U | CENP-R | |||||
rec/% | t1/2/min | rec/% | t1/2/min | rec/% | t1/2/min | rec/% | t1/2/min | rec/% | t1/2/min | |
G1 | 100 | 71±15 | 100 | 77±15 | 90±10 | 57±10 | 100 | 72±15 | 100 | 125±15 |
early S | (45) | - | (70) | - | 59±6 | 118±15 | 100 | 163±40 | 100 | 147±20 |
mid S | (40) | - | 62±6 | 81±5 | 65±6 | 125±30 | 100 | 93±15 | 100 | 160±15 |
late S | 75±15 | 131±30 | 49±10 | 103±10 | 75±8 | 136±15 | 29±2 | 50±8 | 100 | 180±20 |
G2 | (80) | - | 56±8 | 78±5 | 64±6 | 90±14 | 100 | 76±8 | 100 | 137±20 |
rec: fluorescence recovery relative to the initial fluorescence value before bleaching, t1/2: time for half height recovery (in min). Recovery values in brackets: estimated recovery value; for these data, a t1/2 value could not be determined.