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. 2012 Sep 18;7(9):e45043. doi: 10.1371/journal.pone.0045043

Figure 3. The effect of Gö6976 on the phosphorylation of EGFR during FSH-induced FEOs meiotic resumption.

Figure 3

(A) Granulosa cells were collected at the indicated culture time, and analyzed by western blotting. Immunoblots were performed by phospho-EGF receptor antibody, and GADPH was served as an internal standard. Relative intensities were quantified using Gel-pro analyzer 4.0 software. Bars show the mean ± SEM of three independent experiments. Relative levels with a different letter are significantly different (P<0.05). (B) FEOs were cultured in MEM medium supplemented with 0.05 IU/mL FSH, 0.1 µM Gö6976, 10 ng/mL EGF and or 100 ng/mL AR for 16 h, and the rate of GVBD was scored at the end of culture. Data were represented as mean percentage of GVBD ± SEM of three independent experiments. The median number of FEOs in each group was 120. Groups with a different letter are significantly different (P<0.05). C, control; F, FSH; G, Gö6976.