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. 2004 Feb;134(2):813–823. doi: 10.1104/pp.103.029454

Figure 7.

Figure 7.

Hypo-osmotic stress induces a decrease in PIP. Lipids were extracted and analyzed by TLC using an alkaline solvent system. A, Concentration-dependent decrease in PIP. Lane 1, Control. Lanes 2 and 3, 5 and 10 min after the addition of 25% (v/v) water. Lanes 4 and 5, 5 and 10 min after the addition of 50% (v/v) water. Lanes 6 and 7, 5 and 10 min after the addition of 100% (v/v) water. B, Time-dependent decrease in PIP. Lane 1, Control. Lanes 2 to 8, 30 s, 1 min, 2 min, 4 min, 8 min, 15 min, and 30 min after the addition of 100% (v/v) water. C, Time course of PIP decrease after addition of 100% (v/v) water.