Figure 9.
Hyperosmotic stress induces increases in PI(3,5)P2 and PI(4,5)P2. A, Concentration-dependent increases in PI(3,5)P2 and PI(4,5)P2 at 30 min after the start of treatment. Controls, Untreated pollen tubes. B, Time-dependent increases in PI(3,5)P2 and PI(4,5)P2 after addition of 400 mm NaCl. Lipids were extracted and analyzed by TLC using an alkaline solvent system. Lane 1, Control, untreated pollen tubes. Lanes 2 to 8, 30 s, 1 min, 2 min, 4 min, 8 min, 15 min, and 30 min after addition of 400 mm NaCl. Lane 9, Phospholipid marker standard. C, Analysis of time-dependent increases in PI(3,5)P2 and PI(4,5)P2 induced by 400 mm NaCl. Controls, untreated pollen tubes.