Figure 6. The expression of exogenous gene in tumor sections and liver tissues.
(A). Histologic and fluorescence analysis of tumor and liver sections from mice treated with PBS (a, d, g and j), AdCN205-GFP (b, e, h and k), CIK plus AdCN205-GFP (c, f, i and l). a to c, GFP was observed by fluorescence microscopy in the tumor tissue treated with PBS, AdCN205-GFP, or CIK plus AdCN205-GFP. d to f, Immunohistochemical staining for adenoviral hexon protein on the tumor sections (brown staining). g to i, GFP was observed by fluorescence microscopy in liver tissues. j to l, Immunohistochemical staining for adenoviral hexon protein on the liver sections. Original magnification, 200×. (B). The expression of hIL-12 in tumor fraction. Tumor tissues collected at the day of 6 after treatment. The hIL-12 was detected by ELISA. The data was presented as the mean ± SD (n = 3 per group).