Figure 3. Growth of AfIlv3 knockout strains.

A. Radial growth rate analysis of the AfIlv3 strains. 200 spores were spotted onto the centre of a minimal agar plate and incubated at 37°C for seven days. Colony size was measured daily. Each datapoint is the mean of 3 measurements. B. The indicated strains were inoculated onto minimal agar in the absence (-) or presence of 500 µM of the following amino acids: isoleucine (I); valine (V); isoleucine+valine (IV). The plates were incubated at 37°C for 3 days. C. The indicated strains were inoculated onto minimal agar in the presence of 0, 50, 100, 250, 500, 1000 µM isoleucine+leucine+valine. The plates were incubated at 37°C for 3 days. D. 200 spores of the indicated strains were spotted into the centre of a minimal agar plate containing 10 mM nitrate as a sole nitrogen source in the presence of 62.5, 125, 250, 500, 1000 µM isoleucine+leucine+valine. Plates were and incubated at 37°C for 3 days. Images were taken at 20× magnification.