Table II.
Primers for reverse transcriptase-PCR, lengths of amplified products, and comparison of nucleotide sequences of carotenoid biosynthetic genes from Satsuma mandarin with those from Valencia orange, Lisbon lemon, and non-citrus plant species
| cDNA (Accession No.)
|
Sense Primers (Upper) and Antisense Primers (Lower)
|
Length
|
Identity on the Level of Nucleotide Sequencea (Species, Accession No.)
|
|
|---|---|---|---|---|
| Citrus species | Non-citrus species | |||
| bp | % | |||
| CitPSY (AB114648) | ATTCAGCCATTYMGAGAYATG | 503 | 100.0 (Valencia orange, AB114656) | 91.0 (Cucumis melo, Z37543) |
| RAARTTGTTGTARTCATTSGC | 98.5 (Lisbon lemon, AB114664) | 90.3 (Arabidopsis, AY056287) | ||
| CitPDS (AB114649) | CAAGRGATGTTCTWGGTGGA | 545 | 99.2 (Valencia orange, AB114657) | 93.5 (Tageles erecta, AF251014) |
| RTTRCCATCYAARAAKGCCAT | 99.6 (Lisbon lemon, AB114665) | 92.9 (Narcissus pseudonarcissus, X78815) | ||
| CitZDS (AB114650) | RGGHCAYGAGGTGGATATA | 612 | 97.4 (Valencia orange, AB114658) | 93.7 (Pepper, X89897) |
| ATDGGMCCACTCAARTAAAC | 99.5 (Lisbon lemon, AB114666) | 91.1 (N. pseudonarcissus, AJ224683) | ||
| CitCRTISO (AB114651) | AATGCTACMMGMTGGGATAC | 568 | 98.9 (Valencia orange, AB114659) | 84.6 (Tomato, AF416727) |
| WACTATCSCCMACRCARTATA | 99.8 (Lisbon lemon, AB114667) | 83.4 (Arabidopsis, AC011001) | ||
| CitLCYb (AB114652) | TATGGTGTTTGGGTGGATGA | 747 | 99.4 (Valencia orange, AB114660) | 89.8 (Pepper, X86221) |
| DGTCCTTGCYACCATATARC | 100.0 (Lisbon lemon, AB114668) | 89.4 (A. palaestina, AF321534) | ||
| CitHYb (AB114653) | ARRTCVGARMGRTYYACTTAT | 568 | 99.8 (Valencia orange, AB114661) | 92.0 (Pepper, Y09722) |
| CTTCTTCHAVYTCCTTDGGT | 98.7 (Lisbon lemon, AB114669) | 88.6 (T. erecta, AF251018) | ||
| CitZEP (AB114654) | ATWCARATACARAGYAAYGCWT | 984 | 99.5 (Valencia orange, AB114662) | 81.8 (Prunus armeniaca, AF159948) |
| RCCHARRTAWGCYTTGTAAGT | 99.4 (Lisbon lemon, AB114670) | 81.5 (Tomato, Z83835) | ||
| CitLCYe (AB114655) | YACRAAYAAYTAYGGYGTDTG | 651 | 99.8 (Valencia orange, AB114663) | 77.2 (A. palaestina, AF321535) |
| TAHGACCAYTCCTCYTCRTA | 99.5 (Lisbon lemon, AB114671) | 73.4 (Solanum tuberosum, AF321537) | ||
a
Identity between cDNAs was estimated with the exception of nucleotide sequences for primer regions.