Figure 1. MiR-145 expression was down-regulated in ischemia/reperfused heart and H₂O₂-treated NRVMs.

(A), Quantitative real-time PCR showed that miR-145 expression in ischemic myocardium tissues undergoing 1 hour and 3 hours reperfusion was down-regulated, compared with that in the respective sham groups. (n = 10 in each group). (B), Primary neonatal rat ventricle myocytes (NRVM) were treated with 50 µM H₂O₂ for indicated time courses ranging from 0.5 hour to 8 hours. Real-time PCR revealed that miR-145 expression was down-regulated in NRVMs treated with H₂O₂. (C), western blot demonstrated that p53 protein levels were increased at 15 min and 30 min after treatment with H₂O₂, but decreased afterwards. Data are presented as means ± SEM. Significance is indicated as *p<0.05 and **p<0.01, as determined by student’s t test.