Skip to main content
. 2012 Sep 18;7(9):e44673. doi: 10.1371/journal.pone.0044673

Figure 7. The alb1 gene encodes an althiomycin resistance determinant.

Figure 7

A. Activity assays using B. subtilis NRS1473 as the indicator lawn for the producer strains: Db10+VC (S. marcescens Db10 pSUPROM); Db10+alb1 (S. marcescens Db10 pSAN1); Δalb1+VC (SAN2 pSUPROM); Δalb1+alb1 (SAN2 pSAN1) B. RT-PCR analysis of alb1–alb6 transcript levels in each of the alb mutant strains. The template used in the PCR reaction is indicated above the gels as: Db10 (wild type S. marcescens Db10 cDNA); Δ1 (SAN2(Δalb1) cDNA); Δ2 (SAN2 (Δalb2) cDNA); Δ3 (SAN4 (Δalb3) cDNA); Δ4–5 (SAN5 (Δalb4–5) cDNA); Δ6 (SAN60 (Δalb6) cDNA). Reactions were performed in the presence (+) or absence (−) of reverse transcriptase. Con. represents S. marcescens Db10 genomic DNA as a positive control (+) and water as a negative control (−). The primer pairs used to amplify a product internal to a particular gene are indicated to the right of each gel. Twenty five cycles of PCR amplification were used. C. Activity assays using B. subtilis 3610 as the indicator lawn and S. marcescens ATCC274 as the producer strain. D. Activity assays using Db10 (S. marcescens Db10 pSUPROM), Db10 Δalb4–5 (SAN5 pSUPROM) and Db10 PT5-alb1–6 (SAN100 pSUPROM) as the producer strain and using ATCC274+VC (S. marcescens 274 pSUPROM) and ATCC274+alb1 (S. marcescens 274 pSAN1) as the indicator lawn. ‘VC’ represents the empty vector control.