Table II.
Spectrophotometric assays of PDC, KGDC, and BCKDC in three species
Data represent the mean ± se of at least three mitochondria preparations and are expressed as nanomoles NADH per minute per milligram of protein.
Substrate | Pea | Rice | Arabidopsis | |
---|---|---|---|---|
PDC | Pyruvate | 67 ± 5.5 | 83 ± 3.1 | 185 ± 12.5 |
KGDC | α-Ketoglutarate | 40 ± 0.8 | 99 ± 6.7 | 139 ± 9.8 |
BCKDC | α-Ketoisocaproic acid (Leu) | 0 ± 0.4 | 4.5 ± 1.8 | 25 ± 3.1 |
α-Keto-β-methylvaleric acid (Ile) | 0 ± 0.4 | 1.6 ± 0.2 | 21 ± 1.0 | |
α-Ketoisovaleric acid (Val) | 1 ± 0.9 | 0 ± 2.0 | 34 ± 3.2 |