Figure 4.

Effects of NO on elements ratio in the calluses from DR and SR under salt stress. A, Na percentage. B, Ca percentage. C, K to Na ratio. The calluses from DR and SR were cultured on the solid Murashige and Skoog medium (as control). NaCl (200 mm) was contained in the medium for salt stress. One and one-half milliliters of 0.2 mm SNP, 0.3 mm l-NNA, or 0.4 mm 2-phenyl-4,4,5,5-tetramethyl-imidazoline-1-oxyl-3-oxyde (PTIO) was added on the surface of the medium. After 48 h, the calluses were collected. Elements ratio was measured as described in Figure 2. Mean values and se were calculated from three independent experiments. Within each set of experiment, bars with different letters were significantly different at the 0.05 level.