Figure 2. DCF fluorescence used to measure ROS production over time in leaves of intact plants treated with 5 mM MFA (triangles) or 20 µM AA (squares) or control treated (diamonds).

At each time point after inhibitor application, leaves were harvested and incubated with H₂DCFDA. DCF fluorescence was measured in aliquots from the incubation medium as described in ‘Materials and Methods.’ The graphed points are averages of three separate bioreplicate experiments. In each experiment, measurements were made for each of three replicates from plants in independent pots. Error bars show the standard error among the three experiments.