Table 4. Caspase activity of C. albicans cells after H2O2 treatment4.
Strains | 5 mM H2O2 |
HSP90/HSP90 | 72.81±1.32 |
tetO-HSP90/hsp90 | 57.92±1.37* |
HSP90/HSP90+DOX | 71.99±0.48 |
tetO-HSP90/hsp90+DOX | 35.42±1.49* |
After treatment with or without 20 μg/ml DOX for 3 h, caspase activity of C. albicans cells was detected flow cytometry after D2R staining. Three independent experiments were carried out and the results were presented as the mean ± SD of D2R positive cells percentage. * indicated p<0.05 compared with the wild-type strain group.