Fig. 5.

Staurosporine does not affect PDK1 phosphorylation of PKMζ in hippocampal slices. A. Staurosporine (100 nM) applied for 4 hr to the bath of hippocampal slices does not alter the phosphorylation state of the activation loop of PKMζ. Top, representative immunoblots; bottom, group data (between control and staurosporine, PKMζ T410P, p = 0.9; total PKMζ, p = 0.8; n = 5 for each group). B. Staurosporine (100 nM) inhibits phorbol ester-mediated potentiation of synaptic transmission. Unpaired t test, p < 0.05, between PDBu alone (open circles) and PDBu + staurosporine (filled circles) for fEPSPs at 120 min of recording; and paired t test, p < 0.05, comparing before and after PDBu alone, at 10 and 120 min, respectively. Staurosporine has no effect on baseline (one-way ANOVA, repeated measures, followed by Tukey’s post hoc tests, p = 0.8, between 10 and 60 min), and prevents potentiation by PDBu (p = 0.9, between 10 and 120 min; p = 0.8, between 60 and 120 min). Traces above are from the time points denoted in time course below. Bars denote duration of drug application; n = 4.