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. Author manuscript; available in PMC: 2013 Mar 15.
Published in final edited form as: Cancer Res. 2012 Jul 17;72(18):4672–4681. doi: 10.1158/0008-5472.CAN-12-0228

Figure 7. Co-overexpression of AR and constitutively active Kras(G12V) induced epigenetic alterations in normal prostate cells.

Figure 7

Normal prostate basal cells (PEB) were transduced with a lentivus for over-expression of AR, Kras(G12V), or AR+Kras(G12V). A) Protein expression of AR, phospho-Erk, total Erk, EZH2, H3K27Me3, H3K9Me3, and actin (loading control) were measured by Western blot analysis. B) PEB cells transduced with Kras(G12V)+AR were treated with DMSO, 10 μM Erk signaling inhibitor U0126, or 5 μM DZNep for 1 day. The expression levels of total Erk, phospho-Erk, EZH2, and actin were examined by Western blot analysis. The net intensity of EZH2 and actin bands were measured by Carestream Molecular Imaging software (New Haven), and the ratio of EZH2/Actin expression was calculated.