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. Author manuscript; available in PMC: 2013 Sep 15.
Published in final edited form as: Cancer Res. 2012 Aug 3;72(18):4642–4651. doi: 10.1158/0008-5472.CAN-11-3775

Figure 3. B-16-OVA melanoma induces anergy in tumor-infiltrating antigen specific CD4+ T cells.

Figure 3

A. CD4+ T cells from DLN, non-DLN and tumor infiltrating lymphocytes (TIL) were isolated from B16-OVA tumor bearing (5×105 cells) OT-II mice and stimulated with anti-CD3 and anti-CD28 antibodies. IL-2 production was measured by ELISA and is presented as mean±SEM from 3 independent experiments. *P<0.05 (TIL vs. non-DLN). B. CD4+ TIL and T cells from spleens of B16-OVA bearing mice were isolated and immediately lysed for RNA extraction. Grail message levels were detected by real-time PCR. Results are presented as mean±SEM from 3 mice.