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. Author manuscript; available in PMC: 2013 Sep 18.
Published in final edited form as: Anal Chem. 2012 Aug 29;84(18):7638–7642. doi: 10.1021/ac301840f

Fig. 1.

Fig. 1

Separation of Neu5Ac and Neu5Gc. 10 μg of Neu5Ac and Neu5Gc were labeled in 0.1 M 2-AMAC solution in 15% v/v acetic acid in DMSO. (A) Capillary: bare fused silica, total length: 60 cm, effective length: 50 cm, 20 μm ID, applied field: 500 V/cm, normal polarity. BGE: 200 mM boric acid buffer, pH 10. (B) Capillary: neutral coated, total length: 60 cm, effective length: 50 cm, 30 μm ID, applied field: 500 V/cm, reversed polarity. BGE: same as (A). (C) Separation after heat treatment of the sample. Separation conditions are the same as (B). (D) Injection of the heat treated sample. Separation conditions are the same as (B) and (C) except for the BGE: 20 mM ammonium acetate buffer, pH 10. All separations were carried out at 25°C on a Beckman PA800 Plus Instrument.