Table 4B.

Free versus bound aflibercept concentrations

Dose (mg/kg)	Cycle	Aflibercept concentrations (ng/ml)		Free/Bound
		Free	Bound
2.0	Cycle 1, Day 8 (n=3)	6.86 (6.16–7.56)	2.50 (1.76–3.96)	3.87
	Cycle 2, Pretreatment (n=4)	1.50 (0.12–3.43)	3.61 (2.05–6.22)	0.52
	Cycle 5, Pretreatmnt (n=3)	4.20 (1.81–8.01)	8.16 (4.47–13.20)	0.43
2.5	Cycle 1, Day 8 (n=5)	4.96 (3.33–6.02)	2.39 (2.06–2.69)	2.10
	Cycle 2, Pretreatment (n=5)	1.45 (0.99–2.01)	3.35	0.44
	Cycle 5, Pretreatment (n=1)	0.49	4.34	0.11
3.0	Cycle 1, Day 8 (n=4)	7.46 (4.59–9.94)	1.96 (1.78–2.21)	3.88
	Cycle 2, Pretreatment (n=6)	2.20 (0.81–4.15)	3.27 (2.79–3.84)	0.71
	Cycle 5, Pretreatment (n=0)

Values represent mean concentrations with range in parentheses.

^*NOTE: Cycle 1, Day 8- N=3, Cycle 2 Pretreatment- N=5 One patient had bound measurements at each time point, but free measurements only for the Cycle 5, pretreatment timepoint.