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. Author manuscript; available in PMC: 2013 Sep 14.
Published in final edited form as: Biochem Biophys Res Commun. 2012 Aug 10;426(1):12–17. doi: 10.1016/j.bbrc.2012.07.144

Figure 1. Deletion of NPL3 or CBC2 accelerates the senescence of tlc1 mutants, but telomere fragment lengths are comparable between senescing tlc1 and tlc1 npl3 cells.

Figure 1

Population doublings in senescence assays were determined daily for 2x106 cells after 22 hours of growth for sporulated and dissected TLC1/tlc1 Npl3/npl3 (A) or TLC1/tlc1 Npl3/npl3 CBC2/cbc2 cells (B). Error bars represent standard error of the mean, N = 2 – 5. (C) Telomere fragments (indicated by a bracket) from DNA of senescing cultures at specified population doublings (PD) were hybridized with 32P-labeled TG repeats. Ladder marker (in kb) is indicated. (D) Telomere fragment lengths (TFL) from autoradiographs were extrapolated using the Image J software and analyzed. Trend lines indicate average rates of telomere shortening for 10 segregant pairs of tlc1 and tlc1 npl3 mutants.