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. Author manuscript; available in PMC: 2013 Sep 18.
Published in final edited form as: Anal Chem. 2012 Aug 28;84(18):7954–7962. doi: 10.1021/ac301723s

Figure 3.

Figure 3

Tumor cell enrichment using acoustophoretic pre-alignment of cells. RBC lysed blood spiked with prostate cancer cells were processed in the chip. The central outlet cell recovery, i.e. the proportion of cells collected from the central outlet (compared to the total amount of cells collected), was measured by flow cytometry. (A) The effects of cell pre alignment (PA) on PFA fixed DU145 cells (black), and WBCs (red). Acoustic pre alignment; on (solid lines) and off (dashed lines). (B) Separation of three different PFA-fixed prostate cancer cell lines (DU145, PC3, and LNCaP) from blood cells by acoustophoresis with pre-alignment. (C) Acoustic separation of DU145 cells (250,000 / mL) spiked in different concentrations of WBC at an energy level of 120 Vpp2, with active cell PA. (D) Separation of live DU145 cells from blood cells using acoustophoresis with PA. The values given are means, the error bars denoting min and max values.