Effects on cancer cell viability and proliferation. (A) Cell viability was determined by measuring the mitochondrial dehydrogenase activity in three prostate cancer cell lines: DU145, PC3, and LNCaP, 24 h and 48 h after acoustophoresis using transducer voltages of 0, 10, and 20 V. Untreated cells were used as controls and their survival rate was set to 100%. The graphs show the results from at least three separate experiments and the values given are the mean ± SD (n=4). (B) The proliferation of DU145 cells was monitored after acoustophoresis and compared to that of untreated cells. The cells were cultured for four passages of 60 h each. The number of cells at 0 h for each passage was set to 100%. The values given are the mean ± SD (n=4), and n.s. denotes non significant differences.