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. Author manuscript; available in PMC: 2013 Oct 1.
Published in final edited form as: Microbes Infect. 2012 May 29;14(12):1054–1063. doi: 10.1016/j.micinf.2012.05.012

FIG. 2.

FIG. 2

Prophylactic and therapeutic effect of anti-TRP IgG on E. chaffeensis infection in vitro. (A) Cells were treated with IgG (anti-TRP or control) 2 hrs prior to infection and ehrlichial loads determined 1, 2, and 3 days post infection by qPCR. (B) Reduction in ehrlichial load in the presence of anti-TRP32 and anti-TRP120 IgG is protein-specific. Cells were incubated with anti-TRP IgG alone or in combination with recombinant protein (rTRP32 and rTRP120) to neutralize the effect of specific antibody for 2 hrs prior to infection and ehrlichial loads determined by qPCR. (C) Infected cells were treated with IgG (anti-TRP or control) 2 and 24 hrs post-infection, and ehrlichial load determined 3 dpi by qPCR. Bar graphs represent means ± SD, (*p <0.05).