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. 2012 Aug 3;13:92. doi: 10.1186/1471-2202-13-92

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Copyright ©2012 Heermann et al.; licensee BioMed Central Ltd.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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The SCG SC development assay is free of GDNF. Western Blot for GDNF with different dilutions of rat-tail collagen (20μl, 10μl, 5μl, 2.5μl, 1.25μl and 0.6125μl collagen) were analyzed using anti-GDNF antibody specific for human and rat GDNF. Rat adrenal gland (20μg), rat cerebrum (20μg), proteins from undifferentiated PC12 cells (20μg) and recombinant murine GDNF (5ng) were used as positive controls. Gapdh was used a loading control for tissue samples. Whereas recombinant murine GDNF as well as rat GDNF in samples from adrenal gland and cerebrum were detectable, no GDNF signal was obtained in the rat-tail collagen of any amount analyzed.