Table 2.

PCR conditions for various genes

Gene	Product size (bp)	Denaturation	Annealing	Extension	Cycles
IL-1α	229	94°C,1min	55°C, 30s	72°C, 60s	35
IL-1β	305	94°C, 5mins	56°C, 45s	72°C, 60s	35
IL-6	296	95°C, 60s	60°C, 60s	72°C, 60s	35
TNF-α	133	94°C, 1min	61°C, 1min	72°C, 60s	35
IFN-γ	366	95°C, 5mins	56°C, 30s	72°C, 5mins	30
IL-4	195	95°C, 5mins	51°C, 60s	72°C, 60s	35
IL-10	139	94°C, 30s	60°C, 45s	72°C, 60s	35
β-actin	211	94°C, 30s	55°C, 30s	72°C, 60s	30