Figure 5.

Effect of FCM on the protein expression of PPARγ and Runx2 and the mineralization of mouse BMSCs. BMSCs were obtained from the bone marrow of tibiae and femurs collected from 12-week-old C57BL6 mice. (A) After a 48-h treatment with FCM, total proteins were collected, and PPARγ and Runx2 levels were detected by Western blotting. Normal culture medium was used as the control. GAPDH was used as a loading control. (B) BMSCs were cultured for mineralization, during which FCM was added continuously. After induction for 21 days, the mineral nodus was stained by alizarin red. (C) The red stain was quantified by ethylpyridium chloride extraction, which was measured by the absorbance at 550 nm. The results indicated that FCM promoted BMSC mineralization. **P<0.01 compared to control.