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. 2010 Jul 20;1(5):833–839. doi: 10.3892/etm.2010.120

Figure 2.

Figure 2.

Dose-dependence in the stabilization assay of the β-tubulin 5309–318 peptide for the HLA-A*2402 allele. The mean fluorescence intensity (MFI) was recorded at 0.1, 1, 10 and 100 μM of the peptide or DMSO. The MFI increase induced by the β-tubulin 5309–318 peptide compared to DMSO was calculated. Representative results from at least three separate experiments are shown. A PAP-derived peptide at positions 155 to 163 was used as a positive control for binding to the HLA-A*0201, -A*0206 and -A*2402 alleles, but not the other alleles, as reported previously (2). *Statistically significant at P<0.05.