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Effect of fasudil on PGD2-stimulated HSP27 induction in MC3T3-E1 cells. The cultured cells were pretreated with various doses of fasudil for 60 min, and were then stimulated with 10 μM PGD2 or vehicle for 12 h. Extracts of cells were subjected to SDS-PAGE with subsequent Western blot analysis with antibodies against HSP27, HSP70 and GAPDH.
