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Effect of Y27632 on the PGD2-induced phosphorylation of SAPK/JNK in MC3T3-E1 cells. The cultured cells were pretreated with various doses of Y27632 for 60 min and were then stimulated with 10 μM PGD2 or vehicle for 20 min. Extracts of cells were subjected to SDS-PAGE with subsequent Western blot analysis with antibodies against phosphorylated SAPK/JNK and SAPK/JNK. Similar results were obtained with two additional and different cell preparations.
