Fig 3.

5′ RACE analysis to map transcription start sites of A. fumigatus promoters. Total RNA derived from relevant A. fumigatus promoter-reporter gene fusion strains targeted to the pyrG locus was used to generate cDNA. These cDNAs were sequenced using gene-specific primers directing sequencing toward the 5′ end of the transcript. Primers were generated that corresponded to either the native A. fumigatus gene or the firefly luciferase coding sequence. The ATG codons present in the 5′ UTRs from abcA and abcB are shown in bold. The length of each 5′ UTR is indicated at the right. The 5′ untranslated sequences from both the authentic and the fusion genes were identical in all cases tested, except for the gpdA2 promoter. In the case of gpdA2, there were seven additional nucleotides (shown in lower case) found in the transcript produced from the native gene compared to the fusion gene.