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Acetylation of recombinant H4 by S. pombe Hat1. Recombinant H4 was incubated with Hat1 and unlabeled acetyl-CoA. Proteins from the reaction were resolved by electrophoresis and analyzed by Western blotting using antibodies that recognize total H4 (A) or H4 acetylated at K5 and/or K12 (B), K5 (C), K12 (D), or K8 (E). Acetylation at K16 was not detected above background (not shown).
