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. 2012 Sep;11(9):1095–1103. doi: 10.1128/EC.00123-12

Fig 4.

Fig 4

hat1 deletion results in the loss of telomeric silencing in S. pombe. (A) Wild-type and experimental yeast strains were cultured on YEA plates in the presence (5FOA) or absence (YEA) of 5-FOA. Spot cultures represent 5-fold dilutions. Cells were grown for two (YEA) or three (5-FOA) days at 30°C. FY1872, ura4-telomeric marker (tel); KTP36, ura4-tel hat1Δ; KTP24, ura4 disrupted, hat1Δ; LBP6, hat1Δ. (B) Wild-type and experimental yeast strains were cultured on EMMG plates in the presence (EMMG) or absence (EMMG−ura) of added uracil. Spot cultures represent 5-fold dilutions. Cells were grown for two (EMMG) or three (EMMG−ura) days at 30°C. FY1872, ura4-tel; KTP36, ura4-tel hat1Δ; KTP24, ura4 disrupted, hat1Δ; LBP6, hat1Δ.