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Treatment with trichostatin A results in the loss of telomeric silencing. Wild-type (972) and telomeric marker (FY1872) S. pombe strains were cultured in medium containing (+TSA) or lacking (−TSA) trichostatin A and allowed to grow for ∼3 generations at 30°C. Cells were washed and then grown at 30°C in the absence of TSA on EMMG (2 to 3 days), 5-FOA (2 days), and EMMG-ura (4 days). Spot cultures represent 2.5-fold serial dilutions.
