PFOA concentrations in exposed dams. PFOA concentrations were measured in dam serum (A; ng/ml), urine (B; ng/g creatinine), and mammary tissue (C; ng/g tissue weight) on postnatal days (PND) 1, 4, 8 and 18. PFOA concentrations were measured in aspirated milk samples collected on PNDs 2, 8, 11, and 18 (D; ng/ml). Although panels A and C and B and D cannot be directly compared (due to different units), the U-shaped concentration curve present in dam serum (regardless of dose) was also detected in mammary tissue and aspirated milk. Data are shown as mean ± SEM. †Denotes a single reliable measurement at this time due to insufficient volumes in other dams at this dose and time.