Table 1.
Splice events and PTC+ classification
| Tissue | Splice event | Total events | PTC+ events | Percentage PTC+ | Exclusion events | PTC upon exclusion | Inclusion events | PTC upon inclusion |
|---|---|---|---|---|---|---|---|---|
| BMM | Total ES | 730 | 281 | 38% | 428 | 201 | 302 | 80 |
| Single ES | 511 | 310 | 148 | 201 | 80 | |||
| Multiple ES | 219 | 118 | 53 | 101 | - | |||
| A5SS | 130 | 68 | 52% | |||||
| A3SS | 238 | 110 | 46% | |||||
| MXE | 44 | 5 | 11% | |||||
| AFE | 130 | 44 | 34% | |||||
| ALE | 49 | NA | NA | |||||
| Liver | Total ES | 3,102 | 1,285 | 41% | 1,926 | 965 | 1,176 | 320 |
| Single ES | 1,505 | 932 | 531 | 573 | 320 | |||
| Multiple ES | 1,597 | 994 | 434 | 603 | - | |||
| A5SS | 449 | 263 | 59% | |||||
| A3SS | 654 | 370 | 57% | |||||
| MXE | 475 | 66 | 14% | |||||
| AFE | 172 | 66 | 38% | |||||
| ALE | 143 | NA | NA |
Splice isoform classes are shown for both single and multiple exon skipping (ES), alternative 5' splice site (A5SS), alternative 3' splice site (A3SS), mutually exclusive exons (MXE), alternative first exon (AFE) and alternative last exon (ALE). For ALE, no events were classified as PTC+, since the class relies on splicing to two different last exons. Here, a ΔPSI > 20% was required for all classes. NA, not applicable.