Table 1.
Mass spectrometry identification of proteins differentially expressed between wild type MCF7 cells and MCF7 cells stably transfected with 17β-HSD1 (MCF7-17βHSD1).
| Spot | FC | Description | UniProt number | MW exp/ pred |
pI exp |
Pep | Function and/or biological process |
|---|---|---|---|---|---|---|---|
| Spot downregulated in MCF7-17βHSD1 as compared to WT MCF7 | |||||||
| 4183 | 2.7 | Cathepsin D | P07339 | 28/45 | 5.0 | 20 | Proteolysis, pathogenesis of diseases (breast cancer) |
| Ezrin-radixin-moesin-binding phosphoprotein 50 | O14745 | 58/39 | 5.3 | 17 | Wnt signaling pathway | ||
| 3252 | 2.3 | Neudesin | Q9UMX5 | 19/19 | 4.7 | 2 | Neuronal differentiation and proliferation |
| 1703 | 2.2 | Ribonuclease/angiogenin inhibitor 1 (RNH1)(3177)a | P13489 | 58/50 | 4.6 | 24 | Regulation of angiogenesis, mRNA catabolism |
| BRCA2 and CDKN1A interacting protein (BCCIP)a |
Q9P287 | 58/36 | 4.6 | 4 | Promote cell cycle arrest | ||
| Cell division cycle protein 123 homolog | O75794 | 58/39 | 4.6 | 2 | Required for S phase entry of the cell cycle | ||
| Spot unique to WT MCF7 | |||||||
| 2617 | U | Poly(rC)-binding protein 2 | Q15366 | 32/39 | 6.4 | 11 | RNA binding |
| Purine nucleoside phosphorylase | P00491 | 32/32 | 6.4 | 6 | DNA modification | ||
| BTB/POZ domain-containing protein KCTD15 | Q96SI1 | 32/32 | 6.4 | 4 | Potassium ion transport | ||
| RING finger protein 114 | Q9Y508 | 32/26 | 6.4 | 2 | Cell differentiation | ||
| 4335 | U | Peptidyl-prolyl cis-trans isomerase E | Q9UNP9 | 35/33 | 5.4 | 10 | Protein folding, mRNA splicing |
| Transgelin-2 | P37802 | 20/22 | 5.6 | 8 | Muscle development | ||
| Splicing factor, arginine/serine-rich 2 | Q01130 | 35/25 | 5.4 | 2 | mRNA processing | ||
| 3039 | U | RAB11B protein | A5YM50 | 24/25 | 5.7 | 11 | Protein transport, signal transduction |
| Peroxiredoxin-2a | P32119 | 24/22 | 5.7 | 6 | Cell redox regulation, anti- apoptosis |
||
| Splicing factor, arginine/serine-rich 3 | P84103 | 24/19 | 5.7 | 2 | RNA processing in relation with cell proliferation | ||
| Spot upregulated in MCF7-17ΒHSD1 as compared to WT MCF7 | |||||||
| 2714 | 2.5 | Exosome complex exonuclease RRP41 | Q9NPD3 | 29/26 | 6.2 | 3 | rRNA processing |
| Enoyl-CoA hydratase, mitochondrial | P30084 | 29/31 | 6.2 | 6 | Fatty acid and lipid metabolism | ||
| Heat shock 70 kDa protein 1 | P08107 | 29/70 | 6.2 | 5 | Stress response | ||
| Eukaryotic translation initiation factor 4H | Q15056 | 29/27 | 6.2 | 4 | Host-virus interaction, protein biosynthesis | ||
| 2496 | 4.9 | Proliferating cell nuclear antigen (PCNA)a | P12004 | 32/29 | 4.6 | 16 | DNA replication |
| 5474 | 4.0 | Myosin regulatory light chain 2, nonsarcomeric | P19105 | 20/20 | 4.7 | 5 | Cytokinesis, receptor capping, cell locomotion |
| 2300 | 3.3 | 60S acidic ribosomal protein P0(2305) | A8K4Z4 | 40/34 | 5.6 | 6 | Ribosome biogenesis, translation elongation |
| Spot unique to MCF7-17ΒHSD1 | |||||||
| 2305 | U | 17β-hydroxysteroid dehydrogenase type 1(2300) | P14061 | 41/35 | 5.4 | 22 | Steroid biosynthesis |
| 3177 | U | Metastasis inhibition factor nm23 (nm23-H1)a | P15531 | 21/17 | 5.9 | 6 | Cell cycle and proliferation, nucleotide metabolism |
| 4667 | U | 60S ribosomal protein L11 | P62913 | 21/20 | 5.9 | 2 | Binds to 5S ribosomal RNA |
| 4678 | U | S-phase kinase-associated protein 1 (SKP1)a | P63208 | 20/19 | 4.6 | 2 | Ubl conjugation pathway |
The function and/or biological process were obtained from the UniProt database [19].
Spot, spot number; FC, fold change; U, unique; MW, molecular weight (kDa); pI exp, isoelectric point as determined from the two-dimensional gel experiments; Pep, number of unique peptides. aProteins used for RT-qPCR validation. The number after the protein name indicates the additional spot in which the protein was found.