Table 2.
mRNA quantification by RT-qPCR of genes involved in breast cancer cell proliferation within wild type MCF7 and MCF7 cells stably transfected with 17β-HSD1 (MCF7-17βHSD1) and comparison with two-dimensional gel data.
| Descriptiona | MCF7 | MCF7-17βHSD1 | Fold regulationb |
Correlation 2-D gel and RT-qPCR |
|---|---|---|---|---|
| RT-qPCR value (mRNA copies/µg total RNA) | ||||
| Proliferating cell nuclear antigen (PCNA) | 1,599,813 | 4,483,982 | + 2.8 | Yesc |
| Peroxiredoxin 2 | 4,078,760 | 8,585,424 | + 2.1 | Noc |
| Metastasis inhibition factor nm23 (nm23-H1) | 5,366,763 | 19,356,416 | + 3.6 | Yesc |
| S-phase kinase-associated protein 1 (SKP1) | 3,810,452 | 5,714,509 | + 1.5 | Yes |
| BRCA2 and CDKN1A interacting protein (BCCIP) | 345,839 | 1,074,647 | + 3.1 | Noc |
| Ribonuclease/angiogenin inhibitor 1 (RNH1) | 1,015,193 | 727,425 | - 1.4 | Yesc |
Standard deviations were < 10% of duplicates.
aProteins were selected for reverse transcription quantitative real-time polymerase chain reaction (RT-qPCR) after their identification by mass spectrometry analysis of two-dimensional (2-D) gel protein spots.
bFold regulation of mRNA levels in MCF7 cells stably transfected with 17β-HSD1 (MCF7-17βHSD1) compared to wild type MCF7 cells; +, fold increase; -, fold decrease).
cMass spectrometry analysis showed that the two-dimensional spot contained several proteins including the indicated protein.